Simian virus 40 large T overcomes p300 repression of c-Myc

Ghata Singhal; Ravi Kumar Kadeppagari; Natesan Sankar; Bayar Thimmapaya

doi:10.1016/j.virol.2008.04.042

Simian virus 40 large T overcomes p300 repression of c-Myc

Ghata Singhal, Ravi Kumar Kadeppagari, Natesan Sankar, Bayar Thimmapaya^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We previously showed that in quiescent cells p300/CBP negatively regulates the cell cycle G1-S transition by keeping c-Myc in a repressed state and that adenovirus E1A induces c-Myc by binding to p300/CBP. Studies have shown that p300/CBP binding to simian virus 40 large T is indirect and mediated by p53. By using a series of large T mutants that fail to bind to various cellular proteins including p53 as well as cells where p300 is overexpressed or p53 is knocked down, we show that the association of large T with p300 contributes to the induction of c-Myc and the cell cycle. The induction of c-Myc by this mechanism is likely to be important in large T mediated cell cycle induction and cell transformation.

Original language	English (US)
Pages (from-to)	227-232
Number of pages	6
Journal	Virology
Volume	377
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.04.042
State	Published - Aug 1 2008

Funding

We are grateful to Dr. M. K. Rundell for providing some of the LT plasmids used in this study, for helpful discussion and for help in manuscript preparation. Sources of other plasmids used in this study were acknowledged in our previous publications. This work was supported by PHS grant CA74403.

Keywords

Large T
Transcription
c-Myc
p300/CBP
p53

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2008.04.042

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title = "Simian virus 40 large T overcomes p300 repression of c-Myc",

abstract = "We previously showed that in quiescent cells p300/CBP negatively regulates the cell cycle G1-S transition by keeping c-Myc in a repressed state and that adenovirus E1A induces c-Myc by binding to p300/CBP. Studies have shown that p300/CBP binding to simian virus 40 large T is indirect and mediated by p53. By using a series of large T mutants that fail to bind to various cellular proteins including p53 as well as cells where p300 is overexpressed or p53 is knocked down, we show that the association of large T with p300 contributes to the induction of c-Myc and the cell cycle. The induction of c-Myc by this mechanism is likely to be important in large T mediated cell cycle induction and cell transformation.",

keywords = "Large T, Transcription, c-Myc, p300/CBP, p53",

author = "Ghata Singhal and Kadeppagari, {Ravi Kumar} and Natesan Sankar and Bayar Thimmapaya",

note = "Funding Information: We are grateful to Dr. M. K. Rundell for providing some of the LT plasmids used in this study, for helpful discussion and for help in manuscript preparation. Sources of other plasmids used in this study were acknowledged in our previous publications. This work was supported by PHS grant CA74403.",

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doi = "10.1016/j.virol.2008.04.042",

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AU - Kadeppagari, Ravi Kumar

AU - Sankar, Natesan

AU - Thimmapaya, Bayar

N1 - Funding Information: We are grateful to Dr. M. K. Rundell for providing some of the LT plasmids used in this study, for helpful discussion and for help in manuscript preparation. Sources of other plasmids used in this study were acknowledged in our previous publications. This work was supported by PHS grant CA74403.

PY - 2008/8/1

Y1 - 2008/8/1

N2 - We previously showed that in quiescent cells p300/CBP negatively regulates the cell cycle G1-S transition by keeping c-Myc in a repressed state and that adenovirus E1A induces c-Myc by binding to p300/CBP. Studies have shown that p300/CBP binding to simian virus 40 large T is indirect and mediated by p53. By using a series of large T mutants that fail to bind to various cellular proteins including p53 as well as cells where p300 is overexpressed or p53 is knocked down, we show that the association of large T with p300 contributes to the induction of c-Myc and the cell cycle. The induction of c-Myc by this mechanism is likely to be important in large T mediated cell cycle induction and cell transformation.

AB - We previously showed that in quiescent cells p300/CBP negatively regulates the cell cycle G1-S transition by keeping c-Myc in a repressed state and that adenovirus E1A induces c-Myc by binding to p300/CBP. Studies have shown that p300/CBP binding to simian virus 40 large T is indirect and mediated by p53. By using a series of large T mutants that fail to bind to various cellular proteins including p53 as well as cells where p300 is overexpressed or p53 is knocked down, we show that the association of large T with p300 contributes to the induction of c-Myc and the cell cycle. The induction of c-Myc by this mechanism is likely to be important in large T mediated cell cycle induction and cell transformation.

KW - Large T

KW - Transcription

KW - c-Myc

KW - p300/CBP

KW - p53

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Simian virus 40 large T overcomes p300 repression of c-Myc

Abstract

Funding

Keywords

ASJC Scopus subject areas

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