Simian virus 5 membrane protein maturation: Expression in virus-infected cells and from a eukaryotic vector

Hooshmand Sheshberadaran, Robert A. Lamb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Properties of the membrane protein (M) of the paramyxovirus simian virus 5 (SV5) isolated from purified SV5 virions, in SV5-infected cells or when expressed from cDNA using a eukaryotic vector (SV40-M) were examined. Kinetic (pulse-chase radiolabeling) studies showed that M protein expressed in SV5-infected and SV40-M recombinant virus-infected cells underwent maturation, detectable as time-dependent acquisition of reactivity with anti-M protein monoclonal antibodies. Kinetic studies using radiolabeled phosphate and studies with the alkylating agent N-ethylmaleimide indicated that the antigenic maturation of the M protein was not due to phosphorylation or disulfide bond formation, respectively. Immunofluorescent antibody staining studies showed a significant difference in staining patterns between SV40-M recombinant virus-infected cells and SV5-infected cells. SV40-M recombinant virus-infected cells exhibited an intensely staining cytoplasmic fibrillar network, whereas in SV5-infected cells, villar and some small granular structures were the only strongly staining structures.

Original languageEnglish (US)
Pages (from-to)803-809
Number of pages7
JournalVirology
Volume183
Issue number2
DOIs
StatePublished - Aug 1991

Funding

We are deeply indebted to Rick E. Randall drews, St. Andrews, Scotland) for providing tein mAbs. We thank Margaret Shaughnessy assistance. This research was supported

ASJC Scopus subject areas

  • Virology

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