Simian virus 5 membrane protein maturation: Expression in virus-infected cells and from a eukaryotic vector

Hooshmand Sheshberadaran, Robert A. Lamb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Properties of the membrane protein (M) of the paramyxovirus simian virus 5 (SV5) isolated from purified SV5 virions, in SV5-infected cells or when expressed from cDNA using a eukaryotic vector (SV40-M) were examined. Kinetic (pulse-chase radiolabeling) studies showed that M protein expressed in SV5-infected and SV40-M recombinant virus-infected cells underwent maturation, detectable as time-dependent acquisition of reactivity with anti-M protein monoclonal antibodies. Kinetic studies using radiolabeled phosphate and studies with the alkylating agent N-ethylmaleimide indicated that the antigenic maturation of the M protein was not due to phosphorylation or disulfide bond formation, respectively. Immunofluorescent antibody staining studies showed a significant difference in staining patterns between SV40-M recombinant virus-infected cells and SV5-infected cells. SV40-M recombinant virus-infected cells exhibited an intensely staining cytoplasmic fibrillar network, whereas in SV5-infected cells, villar and some small granular structures were the only strongly staining structures.

Original languageEnglish (US)
Pages (from-to)803-809
Number of pages7
JournalVirology
Volume183
Issue number2
DOIs
StatePublished - Aug 1991

ASJC Scopus subject areas

  • Virology

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