TY - JOUR
T1 - Simian virus 5 membrane protein maturation
T2 - Expression in virus-infected cells and from a eukaryotic vector
AU - Sheshberadaran, Hooshmand
AU - Lamb, Robert A.
N1 - Funding Information:
We are deeply indebted to Rick E. Randall drews, St. Andrews, Scotland) for providing tein mAbs. We thank Margaret Shaughnessy assistance. This research was supported
PY - 1991/8
Y1 - 1991/8
N2 - Properties of the membrane protein (M) of the paramyxovirus simian virus 5 (SV5) isolated from purified SV5 virions, in SV5-infected cells or when expressed from cDNA using a eukaryotic vector (SV40-M) were examined. Kinetic (pulse-chase radiolabeling) studies showed that M protein expressed in SV5-infected and SV40-M recombinant virus-infected cells underwent maturation, detectable as time-dependent acquisition of reactivity with anti-M protein monoclonal antibodies. Kinetic studies using radiolabeled phosphate and studies with the alkylating agent N-ethylmaleimide indicated that the antigenic maturation of the M protein was not due to phosphorylation or disulfide bond formation, respectively. Immunofluorescent antibody staining studies showed a significant difference in staining patterns between SV40-M recombinant virus-infected cells and SV5-infected cells. SV40-M recombinant virus-infected cells exhibited an intensely staining cytoplasmic fibrillar network, whereas in SV5-infected cells, villar and some small granular structures were the only strongly staining structures.
AB - Properties of the membrane protein (M) of the paramyxovirus simian virus 5 (SV5) isolated from purified SV5 virions, in SV5-infected cells or when expressed from cDNA using a eukaryotic vector (SV40-M) were examined. Kinetic (pulse-chase radiolabeling) studies showed that M protein expressed in SV5-infected and SV40-M recombinant virus-infected cells underwent maturation, detectable as time-dependent acquisition of reactivity with anti-M protein monoclonal antibodies. Kinetic studies using radiolabeled phosphate and studies with the alkylating agent N-ethylmaleimide indicated that the antigenic maturation of the M protein was not due to phosphorylation or disulfide bond formation, respectively. Immunofluorescent antibody staining studies showed a significant difference in staining patterns between SV40-M recombinant virus-infected cells and SV5-infected cells. SV40-M recombinant virus-infected cells exhibited an intensely staining cytoplasmic fibrillar network, whereas in SV5-infected cells, villar and some small granular structures were the only strongly staining structures.
UR - http://www.scopus.com/inward/record.url?scp=0025826043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025826043&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(91)91015-9
DO - 10.1016/0042-6822(91)91015-9
M3 - Article
C2 - 1853577
AN - SCOPUS:0025826043
VL - 183
SP - 803
EP - 809
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -