Simple purification methods for an αgalactose-specific antibody from chicken eggs

Carrie L. Cook, Winnie Pao, Joseph R. Firca, Byron E. Anderson*, Jonathan Paul Fryer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

For many applications avian antibody from egg yolk (IgY) offers advantages over the well-known mammalian antibodies. Different experimental techniques for the purification of IgY from chickens immunized with an αgalactose-containing antigen (αGal-trisaccharide) were compared. These included ammonium sulfate precipitation, filtration with diatomaceous earth, treatment with deoxycholate, and thiophilic and affinity chromatography. Samples were tested for overall purity, protein and lipid content, and specific activity. Evaluated on the basis of these results and the simplicity of the process, the favored purification method is ammonium sulfate precipitation of diluted egg yolk directly followed by affinity chromatography. The high lipid content of IgY preparations is greatly reduced by either thiophilic or affinity chromatography. Affinity purification of ammonium sulfate precipitated material resulted in anti-αGaltrisaccharide IgY preparations with approximately 1% of the original protein content but approximately 100-fold higher specific activity for the αGal-trisaccharide epitope.

Original languageEnglish (US)
Pages (from-to)305-310
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume91
Issue number3
DOIs
StatePublished - Jan 1 2001

Keywords

  • Affinity chromatography
  • Avian antibody
  • IgY
  • Lipid
  • Thiophilic chromatography
  • αgalactose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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