Simulation and prediction of endothelial cell adhesion modulated by molecular engineering

Adrian Marcin Kopacz*, Wing K Liu, Shu Qian Liu

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Computational modeling is an effective approach for simulating and predicting the development and remodeling of biological systems. Here, we propose to integrate a probabilistic mathematical modeling approach into an emerging bioengineering area known as molecular regenerative engineering. This experimental-computational framework can be used to simulate and predict the efficacy of molecular engineering in controlling and enhancing the regenerative processes of pathologically disordered cells, tissues and organs. We demonstrate the significance and principles of this approach by using a special case as an example: enhancement of endothelial cell adhesion to arterial constructs by siRNA-mediated modulation of an adhesion-inhibiting factor known as Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1. A continuum approach is used to model endothelial cell adhesion at the cellular level, while the Kramers reaction-rate theory is used to model cell adhesion at the subcellular and molecular levels. We show that the proposed computational model can potentially be used to simulate the processes and alterations of endothelial cell adhesion at the molecular and cellular levels under shear flow and to predict the effectiveness of siRNA-mediated SHP-1 knockdown in enhancing endothelial cell adhesion in arterial constructs. These preliminary investigations suggest that this integrated experimental-computational approach may be used for the simulation and prediction of regenerative processes in response to molecular engineering.

Original languageEnglish (US)
Pages (from-to)2340-2352
Number of pages13
JournalComputer Methods in Applied Mechanics and Engineering
Volume197
Issue number25-28
DOIs
StatePublished - Apr 15 2008

Fingerprint

Cell adhesion
Endothelial cells
adhesion
engineering
predictions
simulation
bioengineering
Phosphatases
Biological systems
Shear flow
phosphatases
tyrosine
homology
Reaction rates
cells
shear flow
organs
Adhesion
Modulation
emerging

Keywords

  • Arterial reconstruction
  • Atherosclerosis
  • Endothelial cell adhesion
  • Fluid-structure interaction
  • Immersed finite element method
  • Mesh-free method
  • Reproducing kernel particle method

ASJC Scopus subject areas

  • Computational Mechanics
  • Mechanics of Materials
  • Mechanical Engineering
  • Physics and Astronomy(all)
  • Computer Science Applications

Cite this

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title = "Simulation and prediction of endothelial cell adhesion modulated by molecular engineering",
abstract = "Computational modeling is an effective approach for simulating and predicting the development and remodeling of biological systems. Here, we propose to integrate a probabilistic mathematical modeling approach into an emerging bioengineering area known as molecular regenerative engineering. This experimental-computational framework can be used to simulate and predict the efficacy of molecular engineering in controlling and enhancing the regenerative processes of pathologically disordered cells, tissues and organs. We demonstrate the significance and principles of this approach by using a special case as an example: enhancement of endothelial cell adhesion to arterial constructs by siRNA-mediated modulation of an adhesion-inhibiting factor known as Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1. A continuum approach is used to model endothelial cell adhesion at the cellular level, while the Kramers reaction-rate theory is used to model cell adhesion at the subcellular and molecular levels. We show that the proposed computational model can potentially be used to simulate the processes and alterations of endothelial cell adhesion at the molecular and cellular levels under shear flow and to predict the effectiveness of siRNA-mediated SHP-1 knockdown in enhancing endothelial cell adhesion in arterial constructs. These preliminary investigations suggest that this integrated experimental-computational approach may be used for the simulation and prediction of regenerative processes in response to molecular engineering.",
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Simulation and prediction of endothelial cell adhesion modulated by molecular engineering. / Kopacz, Adrian Marcin; Liu, Wing K; Liu, Shu Qian.

In: Computer Methods in Applied Mechanics and Engineering, Vol. 197, No. 25-28, 15.04.2008, p. 2340-2352.

Research output: Contribution to journalArticle

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