@article{a8d673182be44b3682a4eb8f9a7bbaa9,
title = "Simultaneous cross-evaluation of heterogeneous E. coli datasets via mechanistic simulation",
abstract = "The extensive heterogeneity of biological data poses challenges to analysis and interpretation. Construction of a large-scale mechanistic model of Escherichia coli enabled us to integrate and cross-evaluate a massive, heterogeneous dataset based on measurements reported by various groups over decades. We identified inconsistencies with functional consequences across the data, including that the total output of the ribosomes and RNA polymerases described by data are not sufficient for a cell to reproduce measured doubling times, that measured metabolic parameters are neither fully compatible with each other nor with overall growth, and that essential proteins are absent during the cell cycle-and the cell is robust to this absence. Finally, considering these data as a whole leads to successful predictions of new experimental outcomes, in this case protein half-lives.",
author = "Macklin, {Derek N.} and Ahn-Horst, {Travis A.} and Heejo Choi and Ruggero, {Nicholas A.} and Javier Carrera and Mason, {John C.} and Gwanggyu Sun and Eran Agmon and DeFelice, {Mialy M.} and Inbal Maayan and Keara Lane and Spangler, {Ryan K.} and Gillies, {Taryn E.} and Paull, {Morgan L.} and Sajia Akhter and Bray, {Samuel R.} and Weaver, {Daniel S.} and Keseler, {Ingrid M.} and Karp, {Peter D.} and Morrison, {Jerry H.} and Covert, {Markus W.}",
note = "Funding Information: This work was supported by the Paul G. Allen Frontiers Group through the Allen Discovery Center at Stanford; the Stanford Center for Systems Biology (NIH P50GM107615); an NIH Director's Pioneer Award (5DP1LM01150); an Allen Distinguished Investigator award to M.W.C.; a Stanford Graduate Fellowship, DOE Computational Science Graduate Fellowship (DE-FG02-97ER25308) and Siebel Scholarship to D.N.M.; an NSF Graduate Research Fellowship to N.A.R. and M.L.P.; a Stanford School of Medicine Dean's Postdoctoral Fellowship to J.C.; a Stanford Graduate Fellowship and NSF Graduate Research Fellowship to H.C.; an Agilent Graduate Student Fellowship and Stanford NIST JIMB Training Grant Graduate Fellowship (70NANB15H192) to J.C.M.; and an NIH grant (GM077678) to P.D.K. Publisher Copyright: {\textcopyright} 2020 American Association for the Advancement of Science. All rights reserved.",
year = "2020",
month = jul,
day = "24",
doi = "10.1126/science.aav3751",
language = "English (US)",
volume = "369",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6502",
}