Simultaneous detection of imprinted gene expression (p57 KIP2) and molecular cytogenetics (FICTION) in the evaluation of molar pregnancies

Lori Hoffner, W. Tony Parks, Steven H. Swerdlow, Jason C. Carson, Urvashi Surti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

OBJECTIVE: To simultaneously evaluate the p57 KIP2 antibody expression and genotype of individual cells from paraffin sections of molar pregnancies. STUDY DESIGN: Paraffin sections from 10 typical and unusual molar pregnancies were evaluated with the FICTION technique (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms), using immunofluorescence staining for the p57 KIP2 antibody and enumeration fluorescence in situ hybridization (FISH) probes. The unusual cases included androgenetic/ biparental chimeric complete hydatidiform moles (CHM) and mosaic partial hydatidiform moles (PHM). The unusual molar conceptions provided insight into interpreting atypical p57 KIP2 staining patterns and identifying androgenetic cells. RESULTS: The androgenetic/biparental chimeric CHMs demonstrated a negative p57 KIP2 staining pattern for the androgenetic cells and positive staining for the biparental cells. Concordantly, the FISH results showed delineation between the androgenetic cells and the biparental cells, indicating 2 distinct genotypes. Also, in the 2 cases of mosaic PHM, the partial loss of p57 KIP2 antibody staining was due to mosaic loss of chromosome 11, assumed to be the maternal copy. This provides a biological explanation as to how false interpretation could occur when evaluating p57 KIP2 immunostaining results. CONCLUSION: The FICTION technique is a valuable ancillary tool for simultaneously evaluating the genotype and p57 KIP2 expression in unusual molar pregnancies.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Volume55
Issue number5-6
StatePublished - May 1 2010

Keywords

  • Androgenetic
  • Fiction
  • Molar pregnancy
  • p57 immunostaining

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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