Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy

Xiangyang Zhang*, Minshan Jiang, Amani A. Fawzi, Xiang Li, K. Kirk Shung, Carmen A. Puliafito, Hao F. Zhang, Shuliang Jiao

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.

Original languageEnglish (US)
Pages (from-to)4018-4020
Number of pages3
JournalOptics Letters
Volume35
Issue number23
DOIs
StatePublished - Dec 1 2010

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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