Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain

Christina K. Kim, Samuel J. Yang, Nandini Pichamoorthy, Noah P. Young, Isaac Kauvar, Joshua H. Jennings, Talia N. Lerner, Andre Berndt, Soo Yeun Lee, Charu Ramakrishnan, Thomas J. Davidson, Masatoshi Inoue, Haruhiko Bito, Karl Deisseroth*

*Corresponding author for this work

Research output: Contribution to journalArticle

121 Scopus citations


Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.

Original languageEnglish (US)
Pages (from-to)325-328
Number of pages4
JournalNature Methods
Issue number4
StatePublished - Mar 30 2016

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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