Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by Dual-wavelength photoacoustic microscopy

Li Li, Hao F. Zhang, Roger J. Zemp, Konstantin Maslov, Lihong V. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme β-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 μm. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy.

Original languageEnglish (US)
Pages (from-to)207-215
Number of pages9
JournalJournal of Innovative Optical Health Sciences
Volume1
Issue number2
DOIs
StatePublished - Oct 2008

Keywords

  • Photoacoustic
  • gene expression
  • molecular imaging
  • reporter gene

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Medicine (miscellaneous)
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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