Simultaneous measurement of angular and spectral properties of light scattering for characterization of tissue microarchitecture and its alteration in early precancer

Young L. Kim, Yang Liu, Ramesh K. Wali, Hemant K. Roy, Michael J. Goldberg, Alexey K. Kromin, Kun Chen, Vadim Backman*

*Corresponding author for this work

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

We present a novel instrument to measure the spectral, angular, azimuthal, and polarization dependence of light backscattered by living biological tissues, thus providing the most comprehensive description of the light scattering to obtain unique quantitative information about the microarchitecture of living cells and tissues. We show the potential of this technique to characterize and diagnose early premalignant changes in the epithelia. In studies with a rodent model of colon carcinogenesis, we show that several parameters obtained using this technique, such as the number density of red blood cells in the capillary network immediately underlying the epithelium, the fractal dimension of the tissue, and the average roundness of subcellular structures, are significant for detection of precancerous changes at a very early stage of the carcinogenic process, at which no other histological or molecular markers have been identified.

Original languageEnglish (US)
Pages (from-to)243-256
Number of pages14
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume9
Issue number2
DOIs
StatePublished - Mar 1 2003

Keywords

  • Cancer diagnosis
  • Fractal dimension
  • Light scattering
  • Mie theory
  • Polarization gating

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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