Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

Thomas J. Hefley*, Nancy S. Krieger, Paula H. Stern

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalAnalytical Biochemistry
Volume153
Issue number1
DOIs
StatePublished - Feb 15 1986

Keywords

  • bone formation
  • collagen
  • organ culture
  • peptide hormones
  • prostaglandins
  • protein synthesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria'. Together they form a unique fingerprint.

Cite this