Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

Thomas J. Hefley; Nancy S. Krieger; Paula H. Stern

doi:10.1016/0003-2697(86)90076-X

Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

Thomas J. Hefley^*, Nancy S. Krieger, Paula H. Stern

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E₂. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.

Original language	English (US)
Pages (from-to)	166-171
Number of pages	6
Journal	Analytical Biochemistry
Volume	153
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90076-X
State	Published - Feb 15 1986

Keywords

bone formation
collagen
organ culture
peptide hormones
prostaglandins
protein synthesis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(86)90076-X

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title = "Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria",

abstract = "A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.",

keywords = "bone formation, collagen, organ culture, peptide hormones, prostaglandins, protein synthesis",

author = "Hefley, {Thomas J.} and Krieger, {Nancy S.} and Stern, {Paula H.}",

note = "Funding Information: * This work was supported by NIH Research Grants AM3 1246 and AM 11262.",

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T1 - Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

AU - Hefley, Thomas J.

AU - Krieger, Nancy S.

AU - Stern, Paula H.

N1 - Funding Information: * This work was supported by NIH Research Grants AM3 1246 and AM 11262.

PY - 1986/2/15

Y1 - 1986/2/15

N2 - A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.

AB - A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.

KW - bone formation

KW - collagen

KW - organ culture

KW - peptide hormones

KW - prostaglandins

KW - protein synthesis

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Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

Abstract

Keywords

ASJC Scopus subject areas

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