Abstract
A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.
Original language | English (US) |
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Pages (from-to) | 166-171 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 153 |
Issue number | 1 |
DOIs | |
State | Published - Feb 15 1986 |
Keywords
- bone formation
- collagen
- organ culture
- peptide hormones
- prostaglandins
- protein synthesis
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology