Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG)

Takashi Kusayama; Johnson Wong; Xiao Liu; Wenbo He; Anisiia Doytchinova; Eric A. Robinson; David E. Adams; Lan S. Chen; Shien Fong Lin; Katherine Davoren; Ronald G. Victor; Cheng Cai; Ming Yan Dai; Ying Tian; Pei Zhang; Dereen Ernst; Richard H. Rho; Minglong Chen; Yong Mei Cha; David R. Walega; Thomas H. Everett; Peng Sheng Chen

doi:10.1038/s41596-020-0316-6

Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG)

Takashi Kusayama, Johnson Wong, Xiao Liu, Wenbo He, Anisiia Doytchinova, Eric A. Robinson, David E. Adams, Lan S. Chen, Shien Fong Lin, Katherine Davoren, Ronald G. Victor, Cheng Cai, Ming Yan Dai, Ying Tian, Pei Zhang, Dereen Ernst, Richard H. Rho, Minglong Chen, Yong Mei Cha, David R. WalegaThomas H. Everett, Peng Sheng Chen^*

^*Corresponding author for this work

Anesthesiology

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

neuECG, the simultaneous noninvasive recording of ECG and skin sympathetic nerve activity (SKNA), directly records sympathetic nerve activity over a long period of time. It can be used to measure sympathetic tone in healthy subjects and in subjects with non-cardiovascular diseases. The electrical activity that can be measured on the surface of the skin originates from the heart, the muscle or nerve structures. Because the frequency content of nerve activity falls in a higher frequency range than that of the ECG and myopotential, it is possible to use high-pass or band-pass filtering to specifically isolate the SKNA. neuECG is voltage calibrated and does not require invasive procedures to impale electrodes in nerves and thus has advantages over microneurography. Here, we present a protocol that takes <10 min to set up. The neuECG can be continuously recorded over a 24-h period or longer. We also describe methods to efficiently analyze neuECG from humans using commercially available hardware and software to facilitate adoption of this technology in clinical research.

Original language	English (US)
Pages (from-to)	1853-1877
Number of pages	25
Journal	Nature Protocols
Volume	15
Issue number	5
DOIs	https://doi.org/10.1038/s41596-020-0316-6
State	Published - May 1 2020

Funding

This study was supported in part by NIH Grants R01HL134864 (Y.-M.C.), R01AG049924 (D.R.W.), R42DA043391 (T.H.E.) and R56HL71140, TR002208-01, R01HL139829 and 1OT2OD028190 (P.-S.C.); a Charles Fisch Cardiovascular Research Award endowed by Dr. Suzanne B. Knoebel of the Krannert Institute of Cardiology (T.K. and T.H.E.); a Medtronic-Zipes Endowment and the Indiana University Health-Indiana University School of Medicine Strategic Research Initiative (P.-S.C.); and a Cardiovascular Prospective Award from Mayo Clinic (Y.-M.C.). We thank Matthew D. Podczerwinski of Rose Hulman Ventures for signals shown in Fig. 1a.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41596-020-0316-6

Cite this

Kusayama, T., Wong, J., Liu, X., He, W., Doytchinova, A., Robinson, E. A., Adams, D. E., Chen, L. S., Lin, S. F., Davoren, K., Victor, R. G., Cai, C., Dai, M. Y., Tian, Y., Zhang, P., Ernst, D., Rho, R. H., Chen, M., Cha, Y. M., ... Chen, P. S. (2020). Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG). Nature Protocols, 15(5), 1853-1877. https://doi.org/10.1038/s41596-020-0316-6

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abstract = "neuECG, the simultaneous noninvasive recording of ECG and skin sympathetic nerve activity (SKNA), directly records sympathetic nerve activity over a long period of time. It can be used to measure sympathetic tone in healthy subjects and in subjects with non-cardiovascular diseases. The electrical activity that can be measured on the surface of the skin originates from the heart, the muscle or nerve structures. Because the frequency content of nerve activity falls in a higher frequency range than that of the ECG and myopotential, it is possible to use high-pass or band-pass filtering to specifically isolate the SKNA. neuECG is voltage calibrated and does not require invasive procedures to impale electrodes in nerves and thus has advantages over microneurography. Here, we present a protocol that takes <10 min to set up. The neuECG can be continuously recorded over a 24-h period or longer. We also describe methods to efficiently analyze neuECG from humans using commercially available hardware and software to facilitate adoption of this technology in clinical research.",

author = "Takashi Kusayama and Johnson Wong and Xiao Liu and Wenbo He and Anisiia Doytchinova and Robinson, {Eric A.} and Adams, {David E.} and Chen, {Lan S.} and Lin, {Shien Fong} and Katherine Davoren and Victor, {Ronald G.} and Cheng Cai and Dai, {Ming Yan} and Ying Tian and Pei Zhang and Dereen Ernst and Rho, {Richard H.} and Minglong Chen and Cha, {Yong Mei} and Walega, {David R.} and Everett, {Thomas H.} and Chen, {Peng Sheng}",

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Kusayama, T, Wong, J, Liu, X, He, W, Doytchinova, A, Robinson, EA, Adams, DE, Chen, LS, Lin, SF, Davoren, K, Victor, RG, Cai, C, Dai, MY, Tian, Y, Zhang, P, Ernst, D, Rho, RH, Chen, M, Cha, YM, Walega, DR, Everett, TH & Chen, PS 2020, 'Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG)', Nature Protocols, vol. 15, no. 5, pp. 1853-1877. https://doi.org/10.1038/s41596-020-0316-6

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AU - Zhang, Pei

AU - Ernst, Dereen

AU - Rho, Richard H.

AU - Chen, Minglong

AU - Cha, Yong Mei

AU - Walega, David R.

AU - Everett, Thomas H.

AU - Chen, Peng Sheng

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N2 - neuECG, the simultaneous noninvasive recording of ECG and skin sympathetic nerve activity (SKNA), directly records sympathetic nerve activity over a long period of time. It can be used to measure sympathetic tone in healthy subjects and in subjects with non-cardiovascular diseases. The electrical activity that can be measured on the surface of the skin originates from the heart, the muscle or nerve structures. Because the frequency content of nerve activity falls in a higher frequency range than that of the ECG and myopotential, it is possible to use high-pass or band-pass filtering to specifically isolate the SKNA. neuECG is voltage calibrated and does not require invasive procedures to impale electrodes in nerves and thus has advantages over microneurography. Here, we present a protocol that takes <10 min to set up. The neuECG can be continuously recorded over a 24-h period or longer. We also describe methods to efficiently analyze neuECG from humans using commercially available hardware and software to facilitate adoption of this technology in clinical research.

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Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG)

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

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