Simultaneous x-ray nano-ptychographic and fluorescence microscopy at the bionanoprobe

S. Chen, J. Deng, D. J. Vine, Youssef Nashed, Q. Jin, T. Peterka, Chris Jacobsen, S. Vogt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations


Hard X-ray fluorescence (XRF) microscopy offers unparalleled sensitivity for quantitative analysis of most of the trace elements in biological samples, such as Fe, Cu, and Zn. These trace elements play critical roles in many biological processes. With the advanced nano-focusing optics, nowadays hard X-rays can be focused down to 30 nm or below and can probe trace elements within subcellular compartments. However, XRF imaging does not usually reveal much information on ultrastructure, because the main constituents of biomaterials, i.e. H, C, N, and O, have low fluorescence yield and little absorption contrast at multi-keV X-ray energies. An alternative technique for imaging ultrastructure is ptychography. One can record far-field diffraction patterns from a coherently illuminated sample, and then reconstruct the complex transmission function of the sample. In theory the spatial resolution of ptychography can reach the wavelength limit. In this manuscript, we will describe the implementation of ptychography at the Bionanoprobe (a recently developed hard XRF nanoprobe at the Advanced Photon Source) and demonstrate simultaneous ptychographic and XRF imaging of frozen-hydrated biological whole cells. This method allows locating trace elements within the subcellular structures of biological samples with high spatial resolution. Additionally, both ptychographic and XRF imaging are compatible with tomographic approach for 3D visualization.

Original languageEnglish (US)
Title of host publicationX-Ray Nanoimaging
Subtitle of host publicationInstruments and Methods II
EditorsBarry Lai
ISBN (Electronic)9781628417586
StatePublished - 2015
EventX-Ray Nanoimaging: Instruments and Methods II - San Diego, United States
Duration: Aug 12 2015Aug 13 2015

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


OtherX-Ray Nanoimaging: Instruments and Methods II
Country/TerritoryUnited States
CitySan Diego


  • 3D
  • Hard X-ray fluorescence microscopy
  • Ptychography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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