Single burst study of rec and red mediated recombination in bacteriophage lambda

P. V. Sarthy, M. Meselson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Single bursts from three point crosses of bacteriophage λ were analyzed for recombination of markers several thousand nucleotide pairs apart. Single recombination, mediated by the rec system of Escherichia coli, is usually reciprocal. Double recombinants are also significantly correlated in single bursts, although the correlation is weaker than for reciprocal singles. Reciprocity is not found in crosses mediated by the λ red system. With respect to certain other parameters of recombination, the two systems appear to be alike. Double recombinants usually result from one event, not from two independent single recombinations, and their average clone size is about half that of single recombinants. The results are discussed in terms of current molecular models of recombination.

Original languageEnglish (US)
Pages (from-to)4613-4617
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number12
StatePublished - 1976

ASJC Scopus subject areas

  • General


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