Single Cell Analysis of Proteoforms

Pei Su, Michael A.R. Hollas, Fatma Ayaloglu Butun, Vijaya Lakshmi Kanchustambham, Stanislav Rubakhin, Namrata Ramani, Joseph B. Greer, Bryan P. Early, Ryan T. Fellers, Michael A. Caldwell, Jonathan V. Sweedler, Jared O. Kafader, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.

Original languageEnglish (US)
Pages (from-to)1883-1893
Number of pages11
JournalJournal of Proteome Research
Volume23
Issue number6
DOIs
StatePublished - Jun 7 2024

Keywords

  • individual ion mass spectrometry
  • mass spectrometry
  • nano-DESI
  • proteoform assignment score
  • single cell
  • top-down proteomics

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry

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