Single Cell PCR Coupled with Electrophysiology

T. Tkatch*, D. J. Surmeier

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A fundamental characteristic of the central nervous system is its enormous cellular heterogeneity. Understanding the genetic underpinnings of this diversity demands that we know how gene expression is coordinated in individual neurons and how this pattern of gene expression is translated into neuronal function. One strategy to achieve this goal combines whole cell electrophysiology and reverse transcriptase polymerase chain reaction. Using the patch pipette to both harvest mRNA from an individual neuron and measure neuronal electrical activity, this approach has successfully demonstrated how genes determine function. This article outlines the strengths and weaknesses of this strategy and discusses new methods on the horizon.

Original languageEnglish (US)
Title of host publicationEncyclopedia of Neuroscience
PublisherElsevier Ltd
Pages849-853
Number of pages5
ISBN (Print)9780080450469
DOIs
StatePublished - Jan 1 2009

ASJC Scopus subject areas

  • Neuroscience(all)

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