Single-Cell Tumbling Enables High-Resolution Size Profiling of Retinal Stem Cells

Surath Gomis, Mahmoud Labib, Brenda L.K. Coles, Derek Van Der Kooy*, Edward H. Sargent, Shana O. Kelley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Retinal stem cells (RSCs) are promising candidates for patient-derived cell therapy to repair damage to the eye; however, RSCs are rare in retinal samples and lack validated markers, making cell sorting a significant challenge. Here we report a high-resolution deterministic lateral displacement microfluidic device that profiles RSCs in distinct size populations. Only by developing a chip that promotes cell tumbling do we limit cell deformation through apertured channels and thereby increase the size-sorting resolution of the device. We systematically explore a spectrum of microstructures, including optimized notched pillars, to study and then rationally promote cell tumbling. We find that RSCs exhibit larger diameters than most ciliary epithelial cells, an insight into RSC morphology that allows enrichment from biological samples.

Original languageEnglish (US)
Pages (from-to)34811-34816
Number of pages6
JournalACS Applied Materials and Interfaces
Volume10
Issue number41
DOIs
StatePublished - Oct 17 2018

Keywords

  • cell tumbling
  • ciliary epithelium
  • deterministic lateral displacement
  • label-free
  • microfluidics
  • retinal stem cells
  • size-sorting

ASJC Scopus subject areas

  • Materials Science(all)

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