Abstract
Single-chain variable region (scFv) antibody fragments were used to remove β2-microglobulin (β2m) from the blood of patients. The variable heavy (Vh) and variable light (VI) chains of the anti human β2 monoclonal antibody BBM. 1 were cloned via RT-PCR of the hybridoma cell line. The regeneration ability of the absorbents was tested by stripping the absorbed βm with 0.05 M citrate buffer and repeating the binding capacity measurements. Results show the clinical application of this adsorbent can be economically feasible due the ability to regenerate the adsorption matrix multiple times without significant loss of binding activity.
Original language | English (US) |
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Title of host publication | Transactions - 7th World Biomaterials Congress |
Number of pages | 1 |
State | Published - Dec 1 2004 |
Event | Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: May 17 2004 → May 21 2004 |
Other
Other | Transactions - 7th World Biomaterials Congress |
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Country/Territory | Australia |
City | Sydney |
Period | 5/17/04 → 5/21/04 |
ASJC Scopus subject areas
- General Engineering