Single-chain antibody fragment-based adsorbent for blood purification

C. Daniels*, Guillermo Antonio Ameer

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single-chain variable region (scFv) antibody fragments were used to remove β2-microglobulin (β2m) from the blood of patients. The variable heavy (Vh) and variable light (VI) chains of the anti human β2 monoclonal antibody BBM. 1 were cloned via RT-PCR of the hybridoma cell line. The regeneration ability of the absorbents was tested by stripping the absorbed βm with 0.05 M citrate buffer and repeating the binding capacity measurements. Results show the clinical application of this adsorbent can be economically feasible due the ability to regenerate the adsorption matrix multiple times without significant loss of binding activity.

Original languageEnglish (US)
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
StatePublished - Dec 1 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Daniels, C., & Ameer, G. A. (2004). Single-chain antibody fragment-based adsorbent for blood purification. In Transactions - 7th World Biomaterials Congress