Single-gene transgenic mouse strains for reprogramming adult somatic cells

Bryce W. Carey, Styliani Markoulaki, Caroline Beard, Jacob Hanna, Rudolf Jaenisch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

157 Scopus citations

Abstract

We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.

Original languageEnglish (US)
Pages (from-to)56-59
Number of pages4
JournalNature Methods
Volume7
Issue number1
DOIs
StatePublished - Jan 2010

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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