@article{836bc9e4b4294d8ca359bfeaa74c90a5,
title = "Single-gene transgenic mouse strains for reprogramming adult somatic cells",
abstract = "We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.",
author = "Carey, {Bryce W.} and Styliani Markoulaki and Caroline Beard and Jacob Hanna and Rudolf Jaenisch",
note = "Funding Information: B.W.C. thanks C. Lengner and L. Lee-Houghton for advice and assistance on somatic cell isolation techniques. We thank F. Edenhofer (University of Bonn Medical Center) for a gift of purified recombinant HTN-Cre protein, and J. Dousman and R. Flannery for mouse husbandry. J.H. is supported by a fellowship from the Helen Hay Whitney Foundation. R.J. is supported by grants from the US National Institutes of Health (5-RO1-HDO45022, 5-R37-CA084198 and 5-RO1-CA087869).",
year = "2010",
month = jan,
doi = "10.1038/NMETH.1410",
language = "English (US)",
volume = "7",
pages = "56--59",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "1",
}