Single molecules detection system of optical tweezers

H. T. Chen*, Y. M. Li, L. R. Lou, Z. Gong

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


Using optical tweezers we have developed a biomechanical detection system for single molecules detection. This system can control the sample bead which is used as the handle of single molecules with nanometer precision, it also can make quantitative measurements of displacement with nanometer resolution, and measure the Pico-Newton force involved in the dynamical process of the movement of a single biomolecule. We combined two Donut-Mode optical traps with two high-precision observation setups in this system. Experiments described in this paper demonstrate the performance of the system. In our experiments, we monitored the Brownian motion of polystyrene beads; as a result we can calculate the optical-trap stiffness. In summary, the system can be used in the research of the dynamical motion of a single biomolecule.

Original languageEnglish (US)
Pages (from-to)98-105
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Sep 5 2002
Externally publishedYes
EventNano-Optics and Nano-Structures 2002 - Shanghai, China
Duration: Oct 14 2002Oct 18 2002


  • Donut-mode optical trap
  • Nanometer
  • Pico-Newton
  • Single molecules

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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