Single-nucleotide base excision repair DNA polymerase activity in C. elegans in the absence of DNA polymerase β

Kenjiro Asagoshi, Wade Lehmann, Elena K. Braithwaite, Lucas Santana-Santos, Rajendra Prasad, Jonathan H. Freedman, Bennett Van Houten, Samuel H. Wilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The base excision DNA repair (BER) pathway known to occur in Caenorhabditis elegans has not been well characterized. Even less is known about the DNA polymerase (pol) requirement for the gap-filling step during BER. We now report on characterization of in vitro uracil-DNA initiated BER in C. elegans. The results revealed single-nucleotide (SN) gap-filling DNA polymerase activity and complete BER. The gap-filling polymerase activity was not due to a DNA polymerase β (pol β) homolog, or to another X-family polymerase, since computer-based sequence analyses of the C. elegans genome failed to show a match for a pol β-like gene or other X-family polymerases. Activity gel analysis confirmed the absence of pol β in the C. elegans extract. BER gap-filling polymerase activity was partially inhibited by both dideoxynucleotide and aphidicolin. The results are consistent with a combination of both replicative polymerase(s) and lesion bypass/BER polymerase pol θ contributing to the BER gap-filling synthesis. Involvement of pol θ was confirmed in experiments with extract from pol θ null animals. The presence of the SN BER in C. elegans is supported by these results, despite the absence of a pol β-like enzyme or other X-family polymerase.

Original languageEnglish (US)
Pages (from-to)670-681
Number of pages12
JournalNucleic acids research
Volume40
Issue number2
DOIs
StatePublished - Jan 2012
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Single-nucleotide base excision repair DNA polymerase activity in C. elegans in the absence of DNA polymerase β'. Together they form a unique fingerprint.

Cite this