Single plasmonic nanoparticle tracking studies of solid supported bilayers with ganglioside lipids

Laura B. Sagle, Laura K. Ruvuna, Julia M. Bingham, Chunming Liu, Paul S. Cremer, Richard P. Van Duyne*

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Single-particle tracking experiments were carried out with gold nanoparticle-labeled solid supported lipid bilayers (SLBs) containing increasing concentrations of ganglioside (GM1). The negatively charged nanoparticles electrostatically associate with a small percentage of positively charged lipids (ethyl phosphatidylcholine) in the bilayers. The samples containing no GM1 show random diffusion in 92% of the particles examined with a diffusion constant of 4.3(±4.5) × 10-9 cm2/s. In contrast, samples containing 14% GM1 showed a mixture of particles displaying both random and confined diffusion, with the majority of particles, 62%, showing confined diffusion. Control experiments support the notion that the nanoparticles are not associating with the GM 1 moieties but instead most likely confined to regions in between the GM1 clusters. Analysis of the root-mean-squared displacement plots for all of the data reveals decreasing trends in the confined diffusion constant and diameter of the confining region versus increasing GM1 concentration. In addition, a linearly decreasing trend is observed for the percentage of randomly diffusing particles versus GM1 concentration, which offers a simple, direct way to measure the percolation threshold for this system, which has not previously been measured. The percolation threshold is found to be 22% GM1 and the confining diameter at the percolation threshold only ∼50 nm.

Original languageEnglish (US)
Pages (from-to)15832-15839
Number of pages8
JournalJournal of the American Chemical Society
Volume134
Issue number38
DOIs
StatePublished - Sep 26 2012

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Sagle, L. B., Ruvuna, L. K., Bingham, J. M., Liu, C., Cremer, P. S., & Van Duyne, R. P. (2012). Single plasmonic nanoparticle tracking studies of solid supported bilayers with ganglioside lipids. Journal of the American Chemical Society, 134(38), 15832-15839. https://doi.org/10.1021/ja3054095