Sister chromatid exchange frequency in directly prepared cytotrophoblasts: Demonstration of in vivo deoxyribonucleic acid damage in pregnant women who smoke cigarettes

Lee P. Shulman*, Sherman Elias, Avirachan T. Tharapel, Lirong Li, Owen P. Phillips, Joe Leigh Simpson

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Assessing frequency of sister chromatid exchange is a sensitive method of monitoring exposure to clastogens, mutagens, and other substances that induce deoxyribonucleic acid damage. Aware that cigarette smoke is associated with increased sister chromatid exchange in many cell types, we sought to determine whether an in vivo effect of cigarette smoke could be demonstrated by study of sister chromatid exchange in chorionic villus cells. Directly prepared cytotrophoblasts and cultured mesenchymal core cells were analyzed. Mean sister chromatid exchange frequency in cytotrophoblasts from smoking subjects (8.87 sister chromatid exchanges per cell) was significantly greater than in nonsmoking subjects (5.81 sister chromatid exchanges per cell; p < 0.001); however, no significant difference in cultured mesenchymal core cells was found. Our results demonstrate that maternal exposure to cigarette smoke results in direct placental deoxyribonucleic damage, which in turn could explain deleterious effects of smoking on pregnancy. Increased sister chromatid exchange frequency was observed only in directly prepared cytotrophoblasts, showing the necessity of using this cell type to evaluate the effects of clastogens on placentas.

Original languageEnglish (US)
Pages (from-to)1877-1880
Number of pages4
JournalAmerican journal of obstetrics and gynecology
Volume165
Issue number6 PART 1
DOIs
StatePublished - Dec 1991

Keywords

  • Chorionic villi
  • cigarette smoke
  • prenatal diagnosis
  • sister chromatid exchange

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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