Small angle X-ray scattering data and structure factor fitting for the study of the quaternary structure of the spermidine N-acetyltransferase SpeG

Steven Weigand, Ekaterina V. Filippova, Olga Kiryukhina, Wayne F. Anderson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Here we describe the treatment of the small-angle X-ray Scattering (SAXS) data used during SpeG quaternary structure study as part of the research article "Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG" published in Journal of Molecular Biology [1]. These data were collected on two separate area detectors as separate dilution series of the SpeG and the SpeG with spermine samples along with data from their companion buffers. The data were radially integrated, corrected for incident beam variation, and scaled to absolute units. After subtraction of volume-fraction scaled buffer scattering and division by the SpeG concentration, multiple scattering curves free of an inter-molecular structure factor were derived from the dilution series. Rather than extrapolating to infinite dilution, the structure factor contribution was estimated by fitting to the full set of data provided by dividing the scattering curves of a dilution series by the curve from the most dilute sample in that series.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalData in Brief
Volume6
DOIs
StatePublished - Mar 1 2016

Funding

The SAXS data collection was done at the DND-CAT at the Advanced Photon Source Science User Facility operated for the U.S. Department of Energy (DOE), supported by the U.S. DOE under Contract no. DE-AC02-06CH11357 . This project has been funded with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services , under Contract nos. HHSN272200700058C and HHSN272201200026C (WFA), and the National Science Foundation Grant MCB 1024945 (MAB).

ASJC Scopus subject areas

  • General

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