Snapshot hyperspectral retinal imaging using compact spectral resolving detector array

Hao Li, Wenzhong Liu, Biqin Dong, Joel V. Kaluzny, Amani A. Fawzi*, Hao F. Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Hyperspectral retinal imaging captures the light spectrum from each imaging pixel. It provides spectrally encoded retinal physiological and morphological information, which could potentially benefit diagnosis and therapeutic monitoring of retinal diseases. The key challenges in hyperspectral retinal imaging are how to achieve snapshot imaging to avoid motions between the images from multiple spectral bands, and how to design a compact snapshot imager suitable for clinical use. Here, we developed a compact, snapshot hyperspectral fundus camera for rodents using a novel spectral resolving detector array (SRDA), on which a thin-film Fabry–Perot cavity filter was monolithically fabricated on each imaging pixel. We achieved hyperspectral retinal imaging with 16 wavelength bands (460 to 630 nm) at 20 fps. We also demonstrated false-color vessel contrast enhancement and retinal oxygen saturation (sO2) measurement through spectral analysis. This work could potentially bring hyperspectral retinal imaging from bench to bedside. (Figure presented.).

Original languageEnglish (US)
Pages (from-to)830-839
Number of pages10
JournalJournal of Biophotonics
Issue number6
StatePublished - Jun 2017


  • hyperspectral imaging
  • retinal imaging
  • retinal oxygen saturation

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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