Sodium currents activate without a Hodgkin and Huxley-type delay in central mammalian neurons

Gytis Baranauskas*, Marco Martina

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Hodgkin and Huxley established that sodium currents in the squid giant axons activate after a delay, which is explained by the model of a channel with three identical independent gates that all have to open before the channel can pass current (the HH model). It is assumed that this model can adequately describe the sodium current activation time course in all mammalian central neurons, although there is no experimental evidence to support such a conjecture. We performed high temporal resolution studies of sodium currents gating in three types of central neurons. The results show that, within the tested voltage range from -55 to -35 mV, in all of these neurons, the activation time course of the current could be fit, after a brief delay, with a monoexponential function. The duration of delay from the start of the voltage command to the start of the extrapolated monoexponential fit was much smaller than predicted by the HH model. For example, in prefrontal cortex pyramidal neurons, at -46 mV and 12°C, the observed average delay was 140 μs versus the 740 μs predicted by the two-gate HH model and the 1180 μs predicted by the three-gate HH model. These results can be explained by a model with two closed states and one open state. In this model, the transition between two closed states is approximately five times faster than the transition between the second closed state and the open state. This model captures all major properties of the sodium current activation. In addition, the proposed model reproduces the observed action potential shape more accurately than the traditional HH model.

Original languageEnglish (US)
Pages (from-to)671-684
Number of pages14
JournalJournal of Neuroscience
Volume26
Issue number2
DOIs
StatePublished - Jan 11 2006

Fingerprint

Sodium
Neurons
Decapodiformes
Pyramidal Cells
Prefrontal Cortex
Action Potentials
Axons

Keywords

  • Action potential
  • Activation
  • CA1
  • Dentate gyrus
  • Granule cell
  • Hippocampus
  • Kinetics
  • Prefrontal cortex
  • Pyramidal cell
  • Sodium channel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Sodium currents activate without a Hodgkin and Huxley-type delay in central mammalian neurons",
abstract = "Hodgkin and Huxley established that sodium currents in the squid giant axons activate after a delay, which is explained by the model of a channel with three identical independent gates that all have to open before the channel can pass current (the HH model). It is assumed that this model can adequately describe the sodium current activation time course in all mammalian central neurons, although there is no experimental evidence to support such a conjecture. We performed high temporal resolution studies of sodium currents gating in three types of central neurons. The results show that, within the tested voltage range from -55 to -35 mV, in all of these neurons, the activation time course of the current could be fit, after a brief delay, with a monoexponential function. The duration of delay from the start of the voltage command to the start of the extrapolated monoexponential fit was much smaller than predicted by the HH model. For example, in prefrontal cortex pyramidal neurons, at -46 mV and 12°C, the observed average delay was 140 μs versus the 740 μs predicted by the two-gate HH model and the 1180 μs predicted by the three-gate HH model. These results can be explained by a model with two closed states and one open state. In this model, the transition between two closed states is approximately five times faster than the transition between the second closed state and the open state. This model captures all major properties of the sodium current activation. In addition, the proposed model reproduces the observed action potential shape more accurately than the traditional HH model.",
keywords = "Action potential, Activation, CA1, Dentate gyrus, Granule cell, Hippocampus, Kinetics, Prefrontal cortex, Pyramidal cell, Sodium channel",
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Sodium currents activate without a Hodgkin and Huxley-type delay in central mammalian neurons. / Baranauskas, Gytis; Martina, Marco.

In: Journal of Neuroscience, Vol. 26, No. 2, 11.01.2006, p. 671-684.

Research output: Contribution to journalArticle

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T1 - Sodium currents activate without a Hodgkin and Huxley-type delay in central mammalian neurons

AU - Baranauskas, Gytis

AU - Martina, Marco

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AB - Hodgkin and Huxley established that sodium currents in the squid giant axons activate after a delay, which is explained by the model of a channel with three identical independent gates that all have to open before the channel can pass current (the HH model). It is assumed that this model can adequately describe the sodium current activation time course in all mammalian central neurons, although there is no experimental evidence to support such a conjecture. We performed high temporal resolution studies of sodium currents gating in three types of central neurons. The results show that, within the tested voltage range from -55 to -35 mV, in all of these neurons, the activation time course of the current could be fit, after a brief delay, with a monoexponential function. The duration of delay from the start of the voltage command to the start of the extrapolated monoexponential fit was much smaller than predicted by the HH model. For example, in prefrontal cortex pyramidal neurons, at -46 mV and 12°C, the observed average delay was 140 μs versus the 740 μs predicted by the two-gate HH model and the 1180 μs predicted by the three-gate HH model. These results can be explained by a model with two closed states and one open state. In this model, the transition between two closed states is approximately five times faster than the transition between the second closed state and the open state. This model captures all major properties of the sodium current activation. In addition, the proposed model reproduces the observed action potential shape more accurately than the traditional HH model.

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