To elucidate the role of cGMP in bone resorption, the nucleotide was measured in bone and bone cells in response to several agents including stimulators of bone resorption. In other experiments, sodium nitroprusside (SNP), which elevates bone cGMP, was tested for effects on resorption. In cells from 20-day fetal rat calvaria, cGMP was 52.4 ± 8.4 fmol/106 cells; cAMP was 5.3 ± 0.3 pmol/106 cells. SNP, 0.1 mM, in the presence of IBMX, increased cGMP 74% with no significant effect on cAMP. Parathyroid hormone (PTH), 0.5 μM, did not significantly affect cGMP, but increased cAMP 711%. Calcitriol did not affect either nucleotide. In bone resorption studies, 0.1 mM SNP inhibited the effects of PTH and calcitriol. Lower concentrations of SNP (0.001, 0.01 mM) had no effects on hormone-stimulated resorption. Unstimulated control bones were not affected by 1 nM - 1 mM SNP. The results suggest that elevated cGMP could result in inhibition of bone resorption.
|Original language||English (US)|
|Number of pages||10|
|Journal||Research Communications in Chemical Pathology and Pharmacology|
|State||Published - Jan 1 1992|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)