Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy

J. Maser, A. Osanna, Y. Wang, C. Jacobsen*, J. Kirz, S. Spector, B. Winn, D. Tennant

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomography. J. Microsc. 197, 80-93) or spectroscopic analysis.

Original languageEnglish (US)
Pages (from-to)68-79
Number of pages12
JournalJournal of Microscopy
Volume197
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Cryo microscopy
  • Frozen hydrated
  • Soft X-ray microscopy
  • Spectromicroscopy
  • Synchrotron radiation
  • Zone plate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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