@article{3033be90776447d0a10133c7b42ef786,
title = "Solid-Phase Synthesis of Megamolecules",
abstract = "This paper presents a solid-phase strategy to efficiently assemble multiprotein scaffolds - known as megamolecules - without the need for protecting groups and with precisely defined nanoscale architectures. The megamolecules are assembled through sequential reactions of linkers that present irreversible inhibitors for enzymes and fusion proteins containing the enzyme domains. Here, a fusion protein containing an N-terminal cutinase and a C-terminal SnapTag domain react with an ethyl p-nitrophenyl phosphonate (pNPP) or a chloro-pyrimidine (CP) group, respectively, to give covalent products. By starting with resin beads that are functionalized with benzylguanine, a series of reactions lead to linear, branched, and dendritic structures that are released from the solid support by addition of TEV protease and that have sizes up to approximately 25 nm.",
author = "Kimmel, {Blaise R.} and Modica, {Justin A.} and Kelly Parker and Vinayak Dravid and Milan Mrksich",
note = "Funding Information: B.R.K. was supported by the National Science Foundation Graduate Research Fellowship under Grant DGE-1842165. K.A.P. acknowledges the National Science Foundation Graduate Research Fellowship under Grant DGE-1842165, the Air Force Center of Excellence for Advanced Bioprogrammable Nanomaterials Grant AFRL FA8650-15-2-5518, and the ARO MURI W911NF-18-1-0200. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under Award Number U54CA199091. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This work used facilities of the Integrated Molecular Structure Education and Research Center, which received financial support from the State of Illinois. This work made use of the BioCryo and Keck-II facilities of Northwestern University{\textquoteright}s NU ANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); the MRSEC program (NSF DMR-1720139) at the Materials Research Center; the International Institute for Nanotechnology (IIN); and the State of Illinois, through the IIN. It also made use of the Materials Characterization Facility at Air Force Research Laboratory. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = mar,
day = "11",
doi = "10.1021/jacs.9b12003",
language = "English (US)",
volume = "142",
pages = "4534--4538",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "10",
}