Somatostatin- and substance P-like immunoreactivity in rat neostriatal cultures

Elvira Galarraga, S. T. Kitai, D. James Surmeier*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


In the normal adult neostriatum, somatostatin immunoreactive intemeurons constitute a few percent of the total neuronal population whereas substance P immunoreactive neurons, which project to the substantia nigra, constitute nearly half of the total. Primary monolayer neostriatal cultures derived from E17 rat brains displayed both somatostatin-like and substance P-like immunoreactivity (SOM-IR and SP-IR). However, the proportions of SOM-IR and SP-IR neurons in vitro were significantly different from those in vivo. At 4 days in vitro (DIV), SOM-IR neurons comprised 19% of all neurons and this percentage increased to 30% at 25 DIV. In contrast, SP-IR neurons were less common than expected at 4 DIV (20%) and declined in percentage to 13% at 27 DIV. These results suggest that survival in target-deprived neostriatal cultures is favored for SOM-IR interneurons.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalDevelopmental Neuroscience
Issue number1-2
StatePublished - 1994


  • Cell culture
  • Immunohistochemistry
  • Intemeurons
  • Neostriatum
  • rat

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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