Sonic Hedgehog and BMP2 exert opposing actions on proliferation and differentiation of embryonic neural progenitor cells

Gaofa Zhu*, Mark F. Mehler, Jie Zhao, Shau Yu Yung, John A. Kessler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Although Sonic Hedgehog (Shh) plays a critical role in brain development, its actions on neural progenitor cell proliferation and differentiation have not been clearly defined. Transcripts for the putative Shh-receptor genes patched (Ptc) and smoothened (Smo) are expressed by embryonic, postnatal, and adult progenitor cells, suggesting that Shh can act directly on these cells. The recombinant human amino-terminal fragment of Shh protein (Shh-N) alone did not support the survival of cultured progenitor cells, but treatment with Shh-N in the presence of bFGF increased progenitor cell proliferation. Furthermore, treatment of embryonic rat progenitor cells propagated either in primary culture or after mitogen expansion significantly increased the proportions of both β-tubulin- (neuronal marker) and O4- (oligodendroglial marker) immunoreactive cells and reduced the proportion of nestin- (uncommitted neural progenitor cell marker) immunoreactive cells. By contrast Shh-N had no effect on the elaboration of GFAP- (astroglial marker) immunoreactive cells. Cotreatment with Shh-N and bone morphogenetic protein-2 (BMP2) inhibited the anti-proliferative, astroglial-inductive, and oligodendroglial-suppressive effects of BMP2. Our observations suggest that Shh-N selectively promotes the elaboration of both neuronal and oligodendroglial lineage species and inhibits the effects of BMP2 on progenitor cell proliferation and astroglial differentiation.

Original languageEnglish (US)
Pages (from-to)118-129
Number of pages12
JournalDevelopmental Biology
Volume215
Issue number1
DOIs
StatePublished - Nov 1 1999

Keywords

  • Bone morphogenetic protein
  • Neural lineage development
  • Patched
  • Proliferation
  • Smoothened
  • Sonic Hedgehog

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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