Spatial-analysis of flow-induced intermediate filament displacement

Brian Helmke*, David Thakker, Robert Goldman, Peter Davies

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


Time-lapse optical sectioning microscopy and image restoration was performed on cells expressing green fluorescent protein-vimentin that incorporated into the endogenous intermediate filament (IF) network. Flow onset significantly increased the magnitude and variability in IF movement compared to constitutive motion noted in the absence of shear stress. A newly developed 3-D cross correlation analysis of IF displacement as a function of time and intracellular position demonstrated quantitatively a heterogenous spatial distribution of initial IF movement at the subcellular level. On average, flow induced displacement increased with height in the cell. However, spatial patterns of displacement were highly variable among cells and may be related to factors such as surface topography, network morphology, and cell-cell interactions.

Original languageEnglish (US)
Pages (from-to)S-67-S-68
JournalAnnals of Biomedical Engineering
Issue numberSUPPL. 1
StatePublished - Dec 1 2000
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering


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