Spatial correlations of laminar BOLD and CBV responses to rat whisker stimulation with neuronal activity localized by Fos expression

Hanbing Lu, Sachin Patel, Feng Luo, Shi Jiang Li, Cecilia J. Hillard, B. Douglas Ward, James S. Hyde*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

The spatial relationship between a measured fMRI signal and its underlying neuronal activity remains unclear. One obstacle is the localization of neuronal activity; another is the spatial resolution of fMRI. In the present study, high-resolution BOLD and CBV fMRI experiments (voxel size: 156 x 156 x 2000 μm3) were conducted in the rat whisker barrel cortex at 3 T; neuronal activity across cortical layers was mapped using the Fos expression technique. Results show that BOLD response is weighted by blood volume and that pixels with high BOLD response can be located at the cortical surface or in deep layers, depending on local vasculature. In contrast to BOLD response, the pixels with high CBV response were consistently clustered in the deep cortical layers. Percentage-CBV change in cortical layers IV-V was 7.3 ± 1.5%, which was significantly higher than in layers I-III (4.1 ± 0.9%) and VI (4.3 ± 0.7%) (mean ± SEM). The laminar distribution of CBV response correlates well with neuronal activity localized by Fos expression. We conclude that neuronal activity can be inferred from CBV fMRI data with high spatial accuracy. The data indicate that both intracolumn functional connectivity and neurovascular coupling can be studied using CBV fMRI.

Original languageEnglish (US)
Pages (from-to)1060-1068
Number of pages9
JournalMagnetic resonance in medicine
Volume52
Issue number5
DOIs
StatePublished - Nov 2004
Externally publishedYes

Keywords

  • BOLD
  • CBV
  • Fos expression
  • High-resolution fMRI
  • Whisker barrel cortex

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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