Spatial differences in biologic activity of large uterine leiomyomata

Jian Jun Wei*, Xing Min Zhang, Luis Chiriboga, Herman Yee, Mary A. Perle, Khush Mittal

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Objective: To evaluate the growth pattern of the large uterine leiomyomata (ULM), we examined the spatial gene distributions, vessel density, proliferative activity, and hyaline degeneration. Design: Tissue sections from three-dimensional large ULM, matched myometrium, and small ULM were collected and microarrayed. The spatial difference of the tumor activity was mapped in large ULM. Setting: University clinical research laboratory. Patient(s): Hysterectomy specimens from 7 patients with large (>10 cm) ULM and 3 patients with large (>10 cm) uterine leiomyosarcomas. Intervention(s): Tissue microarray analysis by the immunohistochemistry. Main Outcome Measure(s): Selected gene products, vessel density, and the percentage of hyaline degeneration were all scored in tissue cores/sections of large and small ULM against matched myometrium. Result(s): We found that there was a spherical spatial difference of the tumor activities in large ULM. The tumor region next to the periphery, the most biologically active zone, demonstrated higher levels of gene expression, a higher density of vessels, a higher proliferative rate and a lower level of hyaline degeneration. The large ULM have higher levels of gene products (except for estrogen and progesterone receptors) than small ULM. Conclusion(s): In comparison of the spatial patterns of the gene activity between the large ULM and the large uterine leiomyosarcoma, the large ULM illustrate a growth pattern of nutritional dependence.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalFertility and Sterility
Volume85
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • ER
  • Hypoxia
  • Immunohistochemistry
  • Leiomyosarcoma
  • PR
  • Three dimensions
  • Tissue microarray
  • Uterine leiomyoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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