Spatial memory training induces morphological changes detected by manganese-enhanced MRI in the hippocampal CA3 mossy fiber terminal zone

Binbin Zhang, Kai Hsiang Chuang*, Ci'en Tjio, Way Cherng Chen, Fwu Shan Sheu, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Hippocampal mossy fibers (MFs) can show plasticity of their axon terminal arbor consequent to learning a spatial memory task. Such plasticity is seen as translaminar sprouting from the stratum lucidum (SL) of CA3 into the stratum pyramidale (SP) and the stratum oriens (SO). However, the functional role of this presynaptic remodeling is still obscure. In vivo imaging that allows longitudinal observation of such remodeling could provide a deeper understanding of this presynaptic growth phenomenon as it occurs over time. Here we used manganese-enhanced magnetic resonance imaging (MEMRI), which shows a high-contrast area that co-localizes with the MFs. This technique was applied in the detection of learning-induced MF plasticity in two strains of rats. Quantitative analysis of a series of sections in the rostral dorsal hippocampus showed increases in the CA3a' area in MEMRI of trained Wistar rats consistent with the increased SO + SP area seen in the Timm's staining. MF plasticity was not seen in the trained Lister-Hooded rats in either MEMRI or in Timm's staining. This indicates the potential of MEMRI for revealing neuro-architectures and plasticity of the hippocampal MF system in vivo in longitudinal studies.

Original languageEnglish (US)
Pages (from-to)227-237
Number of pages11
JournalNeuroimage
Volume128
DOIs
StatePublished - Mar 1 2016

Keywords

  • Hippocampal mossy fibers
  • MRI
  • Memory
  • Presynaptic plasticity
  • Spatial learning
  • Water maze

ASJC Scopus subject areas

  • Neurology
  • Cognitive Neuroscience

Fingerprint

Dive into the research topics of 'Spatial memory training induces morphological changes detected by manganese-enhanced MRI in the hippocampal CA3 mossy fiber terminal zone'. Together they form a unique fingerprint.

Cite this