Spatiotemporal visualization of cell membrane dynamics and protein colocalization reveals correlation between membrane dynamics and metastatic invasion

Yue Hou, Lee Cooper, Scott Wilkinson, Adam Marcus, Daniel Brat

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Invasive migration is an important cellular behavior that drives cancer metastasis, which increases the difficulty in treatment and results in poor clinical outcomes. Metastasis is comprised of a series of dynamic processes including cancer cell migration, spreading through circulation system and invasion into distant organs. However, visualization of these underlying steps of metastasis is lack. Although spatiotemporal measurements of cell motion dynamics have been well studied to identify difierent dynamic patterns, little work has been done to visualize how these dynamic patterns occur on migrating cells. Therefore, we develop spatiotemporal visualization tools to broaden the application of the existing dynamical cell analysis. For instance, we propose visualization techniques to classify the cell edge velocities into three states: protrusion, quiescence and retraction and then visualize when and where these three states of membrane dynamics happen on videos of migrating cells. We also create a semi-automatic tool to allow the users to select the ROIs from the correlation map and then plot the ROIs back onto the original cell migration video. These visualization tools help the biologists to better understand the abstract velocity heat maps in a user friendly way. Biologists can also take the advantage of selecting any ROI through these tools, which enables them to easily observe important migration regions. Furthermore, we investigate the correlation between cell membrane dynamics and subcellular proteins colocalization in a cancer model system and discovered a tight coupling between active membrane dynamics and periodic protein colocalizaiton. This active membrane and periodic colocalization pattern correlates with slower and less persistent migration in 3D collagen matrix.

Original languageEnglish (US)
Title of host publicationBCB 2015 - 6th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics
PublisherAssociation for Computing Machinery, Inc
Pages476-477
Number of pages2
ISBN (Electronic)9781450338530
DOIs
StatePublished - Sep 9 2015
Event6th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics, BCB 2015 - Atlanta, United States
Duration: Sep 9 2015Sep 12 2015

Publication series

NameBCB 2015 - 6th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics

Other

Other6th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics, BCB 2015
CountryUnited States
CityAtlanta
Period9/9/159/12/15

Keywords

  • Cancer metastasis
  • Cell migration
  • CellMembrane dynamics
  • Colocalization
  • Spatiotemporal visualization

ASJC Scopus subject areas

  • Software
  • Health Informatics
  • Computer Science Applications
  • Biomedical Engineering

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