Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence

C. Elkins; C. E. Thomas; H. S. Seifert; P. F. Sparling

doi:10.1128/jb.173.12.3911-3913.1991

Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence

C. Elkins^*, C. E. Thomas, H. S. Seifert, P. F. Sparling

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Comment/debate › peer-review

158 Scopus citations

Abstract

Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.

Original language	English (US)
Pages (from-to)	3911-3913
Number of pages	3
Journal	Journal of bacteriology
Volume	173
Issue number	12
DOIs	https://doi.org/10.1128/jb.173.12.3911-3913.1991
State	Published - 1991

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/jb.173.12.3911-3913.1991

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abstract = "Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.",

author = "C. Elkins and Thomas, {C. E.} and Seifert, {H. S.} and Sparling, {P. F.}",

year = "1991",

doi = "10.1128/jb.173.12.3911-3913.1991",

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AU - Elkins, C.

AU - Thomas, C. E.

AU - Seifert, H. S.

AU - Sparling, P. F.

PY - 1991

Y1 - 1991

N2 - Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.

AB - Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.

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U2 - 10.1128/jb.173.12.3911-3913.1991

DO - 10.1128/jb.173.12.3911-3913.1991

M3 - Comment/debate

C2 - 1904861

AN - SCOPUS:0025773014

SN - 0021-9193

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JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 12

ER -

Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence

Abstract

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