Specific β 1 integrins mediate adhesion, migration, and differentiation of neural progenitors derived from the embryonic striatum

Matthew C. Tate, Andrés J. García, Benjamin G. Keselowsky, Michael A. Schumm, David R. Archer, Michelle C. Laplaca*

*Corresponding author for this work

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Early inductive signals within the embryonic mammalian forebrain establish two major germinal regions along the dorsal-ventral axis. The dorsal germinal zone eventually forms the cerebral cortex while the ventral ganglionic eminence primarily forms the striatum and globus pallidus. The mechanisms leading to patterning of specific forebrain structures from these distinct germinal regions are not fully understood but may involve the adhesive and migratory properties of regionally specified cells and their interactions with the extracellular environments in which they reside. In the present study, we isolated ganglionic eminence neural progenitor cells (geNPC), precursors of the adult striatum, from the ventral forebrain germinal zone and analyzed adhesion, migration, and differentiation of geNPC on various extracellular matrix (ECM) substrates in vitro. Specifically, we evaluated the role of β 1 integrins, a family of cell surface receptors important in neural development, in mediating geNPC behavior on ECM molecules expressed in embryonic brain tissue. Adhesion and migration of geNPC were significantly enhanced on laminin (LN) and fibronectin (FN) relative to other ECM substrates. Antibody perturbation experiments revealed that although geNPC express several β 1 integrins (α 1β 1, α 2β 1, α 3β 1, α 5β 1, α 6β 1, α vβ 1), adhesion and migration on LN and FN were primarily mediated by α 6β 1 and α 5β 1, respectively, and these interactions were confirmed by biochemical cross-link/extraction procedures. Finally, neuronal differentiation of geNPC was enhanced on LN, indicating a role for LN in geNPC differentiation. β 1 integrin-ECM interactions may contribute to basic mechanisms of striatal development and may explain the potent migratory capacity of geNPC transplanted into the adult brain.

Original languageEnglish (US)
Pages (from-to)22-31
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume27
Issue number1
DOIs
StatePublished - Sep 1 2004

Fingerprint

Integrins
Stem Cells
Laminin
Extracellular Matrix
Prosencephalon
Fibronectins
Corpus Striatum
Globus Pallidus
Brain
Cell Surface Receptors
Cell Communication
Adhesives
Cerebral Cortex
Cell Differentiation
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Tate, Matthew C. ; García, Andrés J. ; Keselowsky, Benjamin G. ; Schumm, Michael A. ; Archer, David R. ; Laplaca, Michelle C. / Specific β 1 integrins mediate adhesion, migration, and differentiation of neural progenitors derived from the embryonic striatum. In: Molecular and Cellular Neuroscience. 2004 ; Vol. 27, No. 1. pp. 22-31.
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Specific β 1 integrins mediate adhesion, migration, and differentiation of neural progenitors derived from the embryonic striatum. / Tate, Matthew C.; García, Andrés J.; Keselowsky, Benjamin G.; Schumm, Michael A.; Archer, David R.; Laplaca, Michelle C.

In: Molecular and Cellular Neuroscience, Vol. 27, No. 1, 01.09.2004, p. 22-31.

Research output: Contribution to journalArticle

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