Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1

Melanie Stumpf, Xiaojing Yue, Sandra Schmitz, Hervé Luche, Janardan K. Reddy, Tilman Borggrefe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The Mediator complex forms the bridge between transcriptional activators and the RNA polymerase II. Med1 (also known as PBP or TRAP220) is a key component of Mediator that interacts with nuclear hormone receptors and GATA transcription factors. Here, we show dynamic recruitment of GATA-1, TFIIB, Mediator, and RNA polymerase II to the β-globin locus in induced mouse erythoid leukemia cells and in an erythropoietin-inducible hematopoietic progenitor cell line. Using Med1 conditional knockout mice, we demonstrate a specific block in erythroid development but not in myeloid or lymphoid development, highlighted by the complete absence of β-globin gene expression. Thus, Mediator subunit Med1 plays a pivotal role in erythroid development and in β-globin gene activation.

Original languageEnglish (US)
Pages (from-to)21541-21546
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number50
DOIs
StatePublished - Dec 14 2010

Keywords

  • Blood
  • Erythropoiesis
  • Globin
  • Hematopoiesis
  • Transcriptional regulation

ASJC Scopus subject areas

  • General

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