Spectral imaging fluorescence microscopy

Tokuko Haraguchi, Takeshi Shimi, Takako Koujin, Noriyo Hashiguchi, Yasushi Hiraoka*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging fluorescence microscope system, it is also possible to resolve emitted signals from fluorescent dyes that have spectra largely overlapping with each other, such as fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).

Original languageEnglish (US)
Pages (from-to)881-887
Number of pages7
JournalGenes to Cells
Volume7
Issue number9
DOIs
StatePublished - Sep 2002

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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