Spectroscopic analysis beyond the diffraction limit

Biqin Dong, Janel L. Davis, Cheng Sun, Hao F Zhang

Research output: Contribution to journalShort survey

Abstract

The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new capabilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision. It can significantly extend the coding space for multi-molecule super-resolution imaging. Furthermore, it has the potential to detect spectroscopic variations in fluorescence emission associated with molecular interactions, which further enables probing local chemical and biochemical inhomogeneities of the nano-environments. In this review, we seek to explain the working principle of sSMLM technologies and the status of sSMLM techniques towards new super-resolution imaging applications.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalInternational Journal of Biochemistry and Cell Biology
Volume101
DOIs
StatePublished - Aug 1 2018

Fingerprint

Spectroscopic analysis
Diffraction
Molecules
Microscopic examination
Imaging techniques
Single Molecule Imaging
Microscopy
Molecular interactions
Fluorescence
Technology
Optical microscopy
Spectroscopy

Keywords

  • Fluorescence spectral imaging
  • Single-molecule spectroscopy
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

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title = "Spectroscopic analysis beyond the diffraction limit",
abstract = "The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new capabilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision. It can significantly extend the coding space for multi-molecule super-resolution imaging. Furthermore, it has the potential to detect spectroscopic variations in fluorescence emission associated with molecular interactions, which further enables probing local chemical and biochemical inhomogeneities of the nano-environments. In this review, we seek to explain the working principle of sSMLM technologies and the status of sSMLM techniques towards new super-resolution imaging applications.",
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Spectroscopic analysis beyond the diffraction limit. / Dong, Biqin; Davis, Janel L.; Sun, Cheng; Zhang, Hao F.

In: International Journal of Biochemistry and Cell Biology, Vol. 101, 01.08.2018, p. 113-117.

Research output: Contribution to journalShort survey

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AU - Davis, Janel L.

AU - Sun, Cheng

AU - Zhang, Hao F

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AB - The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new capabilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision. It can significantly extend the coding space for multi-molecule super-resolution imaging. Furthermore, it has the potential to detect spectroscopic variations in fluorescence emission associated with molecular interactions, which further enables probing local chemical and biochemical inhomogeneities of the nano-environments. In this review, we seek to explain the working principle of sSMLM technologies and the status of sSMLM techniques towards new super-resolution imaging applications.

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