Spectroscopic Doppler analysis for visible-light optical coherence tomography

Xiao Shu, Wenzhong Liu, Lian Duan, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Retinal oxygen metabolic rate can be effectively measured by visible-light optical coherence tomography (vis-OCT), which simultaneously quantifies oxygen saturation and blood flow rate in retinal vessels through spectroscopic analysis and Doppler measurement, respectively. Doppler OCT relates phase variation between sequential A-lines to the axial flow velocity of the scattering medium. The detectable phase shift is between . π and π due to its periodicity, which limits the maximum measurable unambiguous velocity without phase unwrapping. Using shorter wavelengths, vis-OCT is more vulnerable to phase ambiguity since flow induced phase variation is linearly related to the center wavenumber of the probing light. We eliminated the need for phase unwrapping using spectroscopic Doppler analysis. We split the whole vis-OCT spectrum into a series of narrow subbands and reconstructed vis-OCT images to extract corresponding Doppler phase shifts in all the subbands. Then, we quantified flow velocity by analyzing subband-dependent phase shift using linear regression. In the phantom experiment, we showed that spectroscopic Doppler analysis extended the measurable absolute phase shift range without conducting phase unwrapping. We also tested this method to quantify retinal blood flow in rodents in vivo.

Original languageEnglish (US)
Article number121702
JournalJournal of Biomedical Optics
Volume22
Issue number12
DOIs
StatePublished - Dec 1 2017

Keywords

  • Optical coherence tomography
  • optical Doppler tomography
  • phase variation
  • spectroscopic analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Spectroscopic Doppler analysis for visible-light optical coherence tomography'. Together they form a unique fingerprint.

  • Cite this