TY - JOUR
T1 - Spectroscopic Microvascular Blood Detection From the Endoscopically Normal Colonic Mucosa
T2 - Biomarker for Neoplasia Risk
AU - Roy, Hemant K.
AU - Gomes, Andrew
AU - Turzhitsky, Vladimir
AU - Goldberg, Michael J.
AU - Rogers, Jeremy
AU - Ruderman, Sarah
AU - Young, Kim L.
AU - Kromine, Alex
AU - Brand, Randall E.
AU - Jameel, Mohammed
AU - Vakil, Parmede
AU - Hasabou, Nahla
AU - Backman, Vadim
N1 - Funding Information:
Supported in part by National Institutes of Health grants U01 CA111257, R42CA130508, R01 CA112315, R01 EB003682, R01 CA118794, R01 CA109861, and R01 CA128641, and National Science Foundation grant CBET-0733868. Drs Roy, Goldberg, and Backman are cofounders and shareholders of American BioOptics LLC.
PY - 2008/10
Y1 - 2008/10
N2 - Background & Aims: We previously used a novel biomedical optics technology, 4-dimensional elastically scattered light fingerprinting, to show that in experimental colon carcinogenesis the predysplastic epithelial microvascular blood content is increased markedly. To assess the potential clinical translatability of this putative field effect marker, we characterized the early increase in blood supply (EIBS) in human beings in vivo. Methods: We developed a novel, endoscopically compatible, polarization-gated, spectroscopic probe that was capable of measuring oxygenated and deoxygenated (Dhb) hemoglobin specifically in the mucosal microcirculation through polarization gating. Microvascular blood content was measured in 222 patients from the endoscopically normal cecum, midtransverse colon, and rectum. If a polyp was present, readings were taken from the polyp tissue along with the normal mucosa 10-cm and 30-cm proximal and distal to the lesion. Results: Tissue phantom studies showed that the probe had outstanding accuracy for hemoglobin determination (r2 = 0.99). Augmentation of microvasculature blood content was most pronounced within the most superficial (∼100 μm) layer and dissipated in deeper layers (ie, submucosa). EIBS was detectable within 30 cm from the lesion and the magnitude mirrored adenoma proximity. This occurred for both oxygenated hemoglobin and DHb, with the effect size being slightly greater for DHb. EIBS correlated with adenoma size and was not engendered by nonneoplastic (hyperplastic) polyps. Conclusions: We show, herein, that in vivo microvascular blood content can be measured and provides an accurate marker of field carcinogenesis. This technological/biological advance has numerous potential applications in colorectal cancer screening such as improved polyp detection and risk stratification.
AB - Background & Aims: We previously used a novel biomedical optics technology, 4-dimensional elastically scattered light fingerprinting, to show that in experimental colon carcinogenesis the predysplastic epithelial microvascular blood content is increased markedly. To assess the potential clinical translatability of this putative field effect marker, we characterized the early increase in blood supply (EIBS) in human beings in vivo. Methods: We developed a novel, endoscopically compatible, polarization-gated, spectroscopic probe that was capable of measuring oxygenated and deoxygenated (Dhb) hemoglobin specifically in the mucosal microcirculation through polarization gating. Microvascular blood content was measured in 222 patients from the endoscopically normal cecum, midtransverse colon, and rectum. If a polyp was present, readings were taken from the polyp tissue along with the normal mucosa 10-cm and 30-cm proximal and distal to the lesion. Results: Tissue phantom studies showed that the probe had outstanding accuracy for hemoglobin determination (r2 = 0.99). Augmentation of microvasculature blood content was most pronounced within the most superficial (∼100 μm) layer and dissipated in deeper layers (ie, submucosa). EIBS was detectable within 30 cm from the lesion and the magnitude mirrored adenoma proximity. This occurred for both oxygenated hemoglobin and DHb, with the effect size being slightly greater for DHb. EIBS correlated with adenoma size and was not engendered by nonneoplastic (hyperplastic) polyps. Conclusions: We show, herein, that in vivo microvascular blood content can be measured and provides an accurate marker of field carcinogenesis. This technological/biological advance has numerous potential applications in colorectal cancer screening such as improved polyp detection and risk stratification.
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U2 - 10.1053/j.gastro.2008.06.046
DO - 10.1053/j.gastro.2008.06.046
M3 - Article
C2 - 18722372
AN - SCOPUS:53049107370
SN - 0016-5085
VL - 135
SP - 1069
EP - 1078
JO - Gastroenterology
JF - Gastroenterology
IS - 4
ER -