Spectroscopic single-molecule localization microscopy: applications and prospective

Benjamin Brenner, Cheng Sun, Françisco M. Raymo, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

4 Scopus citations

Abstract

Single-molecule localization microscopy (SMLM) breaks the optical diffraction limit by numerically localizing sparse fluorescence emitters to achieve super-resolution imaging. Spectroscopic SMLM or sSMLM further allows simultaneous spectroscopy and super-resolution imaging of fluorescence molecules. Hence, sSMLM can extract spectral features with single-molecule sensitivity, higher precision, and higher multiplexity than traditional multicolor microscopy modalities. These new capabilities enabled advanced multiplexed and functional cellular imaging applications. While sSMLM suffers from reduced spatial precision compared to conventional SMLM due to splitting photons to form spatial and spectral images, several methods have been reported to mitigate these weaknesses through innovative optical design and image processing techniques. This review summarizes the recent progress in sSMLM, its applications, and our perspective on future work. Graphical Abstract: [Figure not available: see fulltext.]

Original languageEnglish (US)
Article number14
JournalNano Convergence
Volume10
Issue number1
DOIs
StatePublished - Dec 2023

Keywords

  • Polarity sensing
  • Single particle tracking
  • Single-molecule localization microscopy
  • Spectroscopy
  • Super-resolution microscopy

ASJC Scopus subject areas

  • General Engineering
  • General Materials Science

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