Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation

Jessica L. Rouge; Liangliang Hao; Xiaochen A. Wu; William E. Briley; Chad A. Mirkin

doi:10.1021/nn503601s

Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation

Jessica L. Rouge, Liangliang Hao, Xiaochen A. Wu, William E. Briley, Chad A. Mirkin^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

Original language	English (US)
Pages (from-to)	8837-8843
Number of pages	7
Journal	ACS nano
Volume	8
Issue number	9
DOIs	https://doi.org/10.1021/nn503601s
State	Published - Sep 23 2014

Keywords

T4 DNA ligase
enzymatic ligation
gene knockdown
nanoparticle
siRNA

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/nn503601s

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title = "Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation",

abstract = "Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.",

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author = "Rouge, {Jessica L.} and Liangliang Hao and Wu, {Xiaochen A.} and Briley, {William E.} and Mirkin, {Chad A.}",

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AU - Rouge, Jessica L.

AU - Hao, Liangliang

AU - Wu, Xiaochen A.

AU - Briley, William E.

AU - Mirkin, Chad A.

PY - 2014/9/23

Y1 - 2014/9/23

N2 - Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

AB - Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

KW - T4 DNA ligase

KW - enzymatic ligation

KW - gene knockdown

KW - nanoparticle

KW - siRNA

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Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation

Abstract

Keywords

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