Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation

Jessica L. Rouge, Liangliang Hao, Xiaochen A. Wu, William E. Briley, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

Original languageEnglish (US)
Pages (from-to)8837-8843
Number of pages7
JournalACS nano
Volume8
Issue number9
DOIs
StatePublished - Sep 23 2014

Keywords

  • T4 DNA ligase
  • enzymatic ligation
  • gene knockdown
  • nanoparticle
  • siRNA

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA-Nanoparticle Conjugates through Enzymatic Ligation'. Together they form a unique fingerprint.

Cite this