Sphingosine and sphingosine 1-phosphate differentially modulate platelet-derived growth factor-BB-indueed Ca2+ signaling in transformed oligodendrocytes

Alessandro Fatatis*, Richard J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The roles of sphingosine and sphingosine 1-phosphate in Ca2+ signaling following platelet-derived growth factor (PDGF) receptor stimulation were investigated in the oligodendrocyte cell line CEINGE c13, using single-cell fura-2 microfluorimetry and videoimaging. Two different Ca2+ responses were observed, which differed in their delays and kinetics. The first response, which occurred after a shorter delay, exhibited a single Ca2+ peak often followed by a plateau, while the second type of response was characterized by a longer delay and by Ca2+ spikes with different frequencies and amplitudes. The latter phenomenon was never observed after stimulation of G protein-coupled receptors for ATP, ET-1, and BK. The incubation with the inhibitor of sphingosine kinase, DL-threo-dihydrosphingosine, significantly increased the percentage of cells responding to PDGF-BB exposure with Ca2+ spikes (87 versus 47%), while it did not modify the Ca2+ response elicited by exposure to ATP, ET-1, or BK. Exposure to exogenous 10 μM sphingosine or 1 μM sphingosine 1-phosphate produced oscillatory and non-oscillatory Ca2+ responses, respectively, similar to those elicited by PDGF-BB. A second application of PDGF-BB, 30 min after the first, was normally ineffective in producing a Ca2+ response. However, if the second exposure was preceded by the inhibition of sphingosine 1-phosphate formation, an oscillatory Ca2+ response occurred in all cells. We conclude that intracellular levels of sphingosine and sphingosine 1-phosphate may differentially modulate Ca2+ signaling triggered by PDGF receptor stimulation in CEINGE c13-transformed oligodendrocytes.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalJournal of Biological Chemistry
Issue number1
StatePublished - Jan 5 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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