Spinal subarachnoid perfusion of rhesus monkeys

J. A. Kessler, J. D. Fenstermacher, E. S. Owens

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A technique of spinal subarachnoid perfusion is described which effectively isolates the spinal cord from the remainder of the central nervous system. A method of obtaining and analyzing spinal cord tissue after subarachnoid perfusion is presented. The spinal subarachnoid space of 12 rhesus monkeys was perfused with [14C]sucrose, [14]ethylenediaminetetraacetic acid calcium complex (EDTA), and dextran blue as test substances. The sucrose and EDTA spinal cord distribution spaces are 13.6±0.9% in white matter and 18.1±1.4% in grey matter. The diffusion coefficient in tissue of sucrose is 1.7x10-6 cm2/s and of EDTA is 1.55x10-6 cm2/s. The spinal cord tortuosity factor is 2.0-2.1. Six rhesus monkeys were infused intravenously with [3H]sucrose and [14C]EDTA; the spinal capillary exchange half time from blood to extracellular fluid was greater than 6 h for both sucrose and EDTA.

Original languageEnglish (US)
Pages (from-to)614-618
Number of pages5
JournalAmerican Journal of Physiology
Volume230
Issue number3
DOIs
StatePublished - Jan 1 1976

ASJC Scopus subject areas

  • Physiology (medical)

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