Spontaneous gaba-mediated synaptic currents in cerebellar granule cells in culture

Caterina Virginio*, Marco Martina, Enrico Cherubini

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The whole-cell configuration of the patch clamp technique was used to characterize the electrophysiological properties of spontaneous GABA-mediated synaptic currents in cerebellar granule cells grown in a low (5 mM) potassium medium. In the presence of kynurenic acid (1 mM), to block the excitatory drive, bicuculline-sensitive synaptic events were recorded. Their amplitude distribution could be fitted by several Gaussians having the same interpeak distance. In tetrodotoxin (TTX, 1 μM) spontaneous miniature events occurred at a lower frequency. Spontaneous currents reversed polarity at 8.17 ± 0.63 mV, a potential close to Eci; the decay phase could be fitted with a single exponential having at - 60 mV a time constant of 48.7 ± 1.5 ms. In low noise recordings, channel closing could be resolved during the decay phase of miniature events. It appeared that a single quantum of GABA opened few channels on the postsynaptic membrane.

Original languageEnglish (US)
Pages (from-to)1285-1289
Number of pages5
JournalNeuroReport
Volume6
Issue number9
DOIs
StatePublished - Jan 1 1995

Keywords

  • Cerebellum
  • GABA
  • Granule cells
  • Spontaneous inhibitory postsynaptic currents
  • Whole cell and single channel

ASJC Scopus subject areas

  • Neuroscience(all)

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