Stability of oligodeoxynucleotide complexes with cationic phospholipid and peptide carriers

B. G. Tenchov, R. Koynova, R. W. Ahn, Thomas V O'Halloran, R. C. MacDonald

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The design of efficient cationic lipid and peptide nanocarriers for oligodeoxynucleotide (ODN) delivery into cells requires precise understanding and control of their physical properties. In this work we used X-ray diffraction, fluorescence resonance energy transfer (FRET), dynamic light scattering (DLS) and zeta-potential measurements to characterize the stability of the nanoparticles formed by ODN with cationic phospholipids and with a native cationic peptide, protamine. The most important findings can be summarized as follows: 1) ODNs form stable complexes with protamine and cationic phospholipids at salt concentrations below ~0.5 and ~0.3 M NaCl, respectively. 2) The ODN lipoplexes are typified by tightly packed lamellar phases and disordered ODN arrangement between the lipid bilayers. 3) ODN/protamine complexes with positive zeta-potentials gradually increase in size in the course of hours and days, while the size of nanoparticles with negative zeta-potentials remains constant. 4) There are significant differences between ODN/lipid and ODN/protamine particle size distributions. The former displayed a characteristic maximum at cationic/anionic 1:1 charge ratio, while the latter were not influenced by variations of the ODN/protamine ratio.

Original languageEnglish (US)
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages342-345
Number of pages4
Volume3
StatePublished - Nov 9 2010
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Other

OtherNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim, CA
Period6/21/106/24/10

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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