Stabilization of wild-type p53 in human T-lymphocytes transformed by HTLV-I

Robert L. Reid, Paul F. Lindholm, Alidad Mireskandari, Jürgen Dittmer, John N. Brady

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Adult T-cell leukemia/lymphoma is an aggressive malignancy associated with infection by the human T-lymphotropic virus type-I (HTLV-I). We now demonstrate that p53 expression is elevated in the HTLV-I-transformed T-lymphocyte lines C81, MT-2, MT-4 and HUT 102. In pulse-chase experiments, the p53 protein demonstrated a prolonged half-life of 2 to 8 h in HTLV-I-transformed cells compared with 0.5 to 1.0 h for wildtype p53 in primary human and murine fibroblasts, or human peripheral blood lymphocytes. In cell lines C81 and HUT 102, which exhibited the longest p53 protein half-life, the wild-type-related PAb1620 epitope was detected at reduced levels. The PAb240 mutant-related p53 epitope was not detected in any of the transformed cell lines. By direct sequence analysis of RT-PCR products, the entire p53 cDNA coding sequence was determined to be wild-type in all four cell lines. Stabilization of wild-type p53 may represent its functional inactivation and contribute to lymphocyte transformation by HTLV-I.

Original languageEnglish (US)
Pages (from-to)3029-3036
Number of pages8
JournalOncogene
Volume8
Issue number11
StatePublished - 1993

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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